The oocytes have been fertilised, and we now have presumptive zygotes! How can we give these zygotes the best opportunity to develop into competent blastocysts, ready to be cryopreserved or transferred into recipient cattle? Here are some of our tips following IVF: 1. Remove cumulus cells quickly and thoroughly to reduce the metabolic requirements on […]
Tips following IVF. Read More »
When handling oocytes, temperature and pH fluctuations should be minimised. Therefore it is recommended to use incubators, heated stages and plates to warm all medium and consumables.
Ovum Pick Up (OPU) and In Vitro Maturation (IVM) Read More »
Ensure your consumables are “embryo-safe”by using Mouse Embryo Assay (MEA) and/or Bovine Embryo Assay (BEA) approved consumables.
Contaminants can be introduced into your laboratory through new batches of oils, media and general plasticware which is why MEAs and BEAs can provide quality assurance.
The Importance of Embryo Assay Testing Your Laboratory Consumables Read More »
Regular maintenance of your laboratory and it’s equipment is important. Maintenance includes calibrating equipment regularly (daily if possible otherwise a couple times a week), keeping track of batches of media, culture oils and laboratory consumables being used and being aware of potential sources of volatile organic compounds (VOCs).
Looking for IVF laboratory management tips? Keep reading. Read More »
There are many advantages and disadvantages to choosing the right method of embryo production for your cattle as both technologies have their place.
Ovum Pick Up (OPU) vs Multiple Ovulation Embryo Transfer (MOET) Read More »
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