The most accurate way to measure the fertilisation ability of semen in vitro is to test it using abattoir-derived cumulus-oocyte-complexes (COCs). While often sperm has been assessed for motility and morphology when collected and cryopreserved, sometimes internal issues (eg. DNA fragmentation) that cannot be visualised by microscopy can severely impact IVF results. These results can then be used by producers to finalise in vitro mating’s of commercially collected COC/oocytes from their valuable donor cows and heifers. It will also assist in the preparation of enough semen straws per bull at the time of IVF. Taking the time to test semen
When handling oocytes, temperature and pH fluctuations should be minimised. Therefore it is recommended to use incubators, heated stages and plates to warm all medium and consumables. We recommend these items should be calibrated to 38.5°C. Using an appropriately buffered medium will limit pH fluctuations which is why we have designed our entire media suite to have exceptional pH stability due to the combination of buffers that we use. We also have transport maturation media options that can be used without the requirement of gas during in vitro maturation (IVM). Transporting gas cylinders across the country can be logistically challenging
Ensure your consumables are “embryo-safe”by using Mouse Embryo Assay (MEA) and/or Bovine Embryo Assay (BEA) approved consumables. Contaminants can be introduced into your laboratory through new batches of oils, media and general plasticware which is why MEAs and BEAs can provide quality assurance. You will notice that many IVF laboratory products available for purchase are already validated by a MEA. However, for unvalidated products you may like to arrange a MEA and/or BEA to confirm batch quality. Contamination of oils can cause considerable issues for your embryo results. Many IVF laboratories use micro drops under oil for embryo production, to
Last week we celebrated Jeremy’s academic career which has led him to be in the top 1% of cited researchers for his field across the world! During his Academic career he has been published over 290 times with his focus being the developmental capacity of mammalian oocytes and early embryos. We congratulate Jeremy on his academic success and the significance it has on the cattle breeding industry. Jeremy will continue his endeavours as Founder & Chief Scientific Officer of ART Lab Solutions and Chief Scientific Officer of Fertilis.
It’s always exciting to have trainees come through and learn quality control measures of an IVF laboratory. Additionally, the team learnt the theory and handling techniques of COC/oocyte maturation, in vitro fertilisation, in vitro culture and cryopreservation. Thank you for being an inspiring bunch! If you are interested in upskilling your laboratory skills within the reproductive space, get in touch with our team at email@example.com
Regular maintenance of your laboratory and it’s equipment is important. Maintenance includes calibrating equipment regularly (daily if possible otherwise a couple times a week), keeping track of batches of media, culture oils and laboratory consumables being used and being aware of potential sources of volatile organic compounds (VOCs). While some of this tracking can seem mundane and unnecessary when things are going well, you will be glad you did when or if you need to troubleshoot when things aren’t going so great. INCUBATORS All CO2 incubators should be regularly calibrated for both CO2 and temperature. Benchtop incubators should also be